Urine Collection Guidelines for Drug Testing
The following are based on the Mandatory Guidelines for Federal Workplace Drug Testing Programs. For testing programs that are not Federally Regulated you may choose to model your collection procedures after some or all of these time tested methods. Some States also have requirements for drug testing, which you should consult prior setting up your testing program. You may contact MD Technical Support at 866-933-0964 for information about your State.

  • Provide a restroom/stall/portable toilet with adequate privacy, and ensure that access by the general public is limited during the procedure. Ensure that undetected access (e.g., through a door not in your view) is not possible.

  • Ensure proper identification of the donor. (either via picture ID or donor is known by the collector).

  • Secure water sources or otherwise make them unavailable to the donor (ie. Turn off water or secure handles and tank lid with tamper proof tape, put bluing agent in toilet and tank).

    TIP: The cheapest and easiest way make a bluing agent is to by blue food coloring (Adams Extract" is a common brand) at you local grocery store. Clean out an empty clear shampoo or water bottle with a squeeze spout. Fill it with water and add several drops of blue coloring and shake until you get the color you want. A few squirts in the toilet will turn the water blue. Bluing Tablets are also available to purchase.

  • Inspect the site to ensure that no unauthorized substances are present. (ie. no cleaning agents, soap, etc.) and secure areas and items (e.g., ledges, trash cans, under sink areas, paper towel holders) that appear suitable for concealing contaminants.

  • Ask donor to remove any unnecessary clothing (eg. coats, hats, purses, etc.). Providing a locked area or receipt of personal items may help ensure that personal items are kept secure.

  • Ask donor to empty pockets. If nothing is there which could be used to adulterate the sample, the donor may place items back in pocket. If donor refuses to empty pockets this can be considered a refusal to cooperate in the testing process.

  • Instruct donor to wash and dry hands prior to collection, but further access to water may not be allowed. Liquid soap is recommended. A solid bar of soap gives donor a chance to conceal soap shavings under his/her fingernail and subsequently use them to attempt to adulterate the specimen. Access to a sink area near the bathroom being used for the collection can be helpful, so the collector is not constantly securing and un-securing a sink water source for hand washing. An alternative to soap/water for hand washing after (not prior hand washing) is the quick drying anti bacterial hand washing gel.

  • Ensure access to collection materials, test devices, and specimen’s is effectively restricted.

  • Inform donor of minimum specimen level. Generally speaking 30 mL is sufficient.

  • Write donor name, ID #, and date in the designated area on the collection container and give to donor.

  • Set a reasonable time limit for the donor to be inside the restroom.

  • Upon receiving the specimen from the donor, place security seal over collection container, check the specimen volume, temperature, and for adulteration or substitution. Do not wait longer than 4 minutes to check the temperature after receiving the specimen. The acceptable temperature range is 90 - 100 degrees F.

  • Inspect for unusual color, presence of foreign objects or material, or other signs of adulteration (e.g., specimen is blue, foaming when shaken, smells of bleach).

  • Any sample suspected of being adulterated should be considered INVALID and another sample should be collected. Direct observation of the second collection should be considered. If they are unable to provide another sample immediately, start Shy Bladder Procedure.
Shy Bladder Procedure (donor is unable to provide a sample)
  • First, begin the process. Ask them to try. Explain that most people can provide 30 mL of urine even when they think they can’t.

  • If they demonstrate inability to provide a sample, note the time. Tell them they have up to 3 hours to provide a sample. Encourage donor to drink up to 40 oz. of water while waiting.

  • Do not combine urine collected from separate attempts to create one specimen of sufficient volume.

  • If unable to provide a sample in 3 hours, discontinue the collection. This may require an immediate medical evaluation ( explaining this during the process may provide the motivation to provide a specimen).
Disposal of Urine, Collection Cups, and Rapid Testing Devices
  • Urine and Saliva are not considered carriers of blood borne pathogens, and not considered bio hazardous waste, and therefore regulations regarding medical waste are not applicable. That said, Universal Precautions, should always be practiced (ie. gloves, and other PPE). Urine and saliva samples and containers may be discarded in the regular trash. It is recommended that you double bag trash cans used for this purpose for extra caution and sanitary purposes.

  • It is recommended that you always conduct a rapid drug test immediately after the collection and in the continued presence of the donor. This accomplishes 2 things:

    • It maintains chain of custody of the sample with the donor. Additionally, if your policy calls for confirmation testing of a positive screening result, having the donor present for the completion of laboratory forms, etc. is an added convenience for the collector and donor. If the sample isn’t sealed until after the rapid test is performed the donor should also be present.

    • After the test is performed and results are recorded if there is no additional need for the sample the collector may instruct the donor to return the sample to restroom and dump the urine in the toilet, flush, and throw the collection cup into a designated trash can. This further minimizes urine exposure to the collector.
For Assistance setting up your Collection Procedures call
Micro Distributing Technical Support at 866-933-0964